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Briefly, in the NADH-coupled assay, 14 g (1.2 M) of detergent-purified protein was added to 150 L of reaction buffer containing 50 mM HEPES-KOH pH 8.0, 60 g/mL pyruvate kinase (Roche), 16 mg/mL lactate dehydrogenase (Roche), 4 mM phosphoenolpyruvate (Roche), 0.3 mM NADH, 3 mM ATP, 10 mM MgCl, 0.01% (w/v) LMNG, and 0.001% (w/v) CHS