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Because receiving vitamin D shots cuts out any absorption issues, its a highly recommended form of vitamin therapy for people who are at risk for a deficiency or have conditions that could benefit from more vitamin D intake

Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

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