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Genome-wide assessment of differential DNA methylation associated with autoantibody production in systemic lupus erythematosus

The cell pellet was lysed in 1 ml of lysis buffer containing 10 mM Tris HCl (pH 7.5), 150 mM NaCl, 5 mM EDTA, 0.5% Triton X-100, 1 mM phenylmethylsulfonyl fluoride, and 1 mM dithiothreitol (DTT), sonicated at 4 C using ultrasonic homogenizer Q700 (QSONICA), and then centrifuged at 17,200 g for 5 min at 4 C

Lymph protects metastasizing melanoma cells from ferroptosis

In addition to the systemic differences between rodents and humans, several mechanistic constraints further reduce the likelihood that GLP-1secreting engineered probiotics will succeed clinically