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8 Future directions and research gaps Despite the increasing body of evidence that elucidates the synergistic interactions of CIT, leucine, and physical exercise on the enhancement of muscle and vascular health in the aging population, several methodological limitations persist within the extant literature

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After reaching 95% confluence, the cells were induced to differentiate using DMEM with 10% FBS, 0.5 g ml 1 insulin, 1 nM T3, 0.125 mM indomethacin, 2 g ml 1 dexamethasone, 0.5 M IBMX and P/S (day 0)