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Although we currently do not know the time required for vesicle reuse, recent studies using hippocampal synapses as well as cerebellar mossy fiber terminals in slices (Watanabe et al., A suggestion of rapid reuse of incompletely filled vesicles comes from experiments in which vesicular glutamate transport was abolished by dialyzing presynaptic terminals with a glutamate-free pipet solution, while EPSCs were continuously monitored (Hori and Takahashi, + buildup of endocytosed vesicles with strong buffers, results in rapid synaptic depression in hippocampal preparations (Ertunc et al., A second indication comes from an experiment in which the input-output relationship of presynaptic and postsynaptic AP firings is monitored, while vesicular glutamate transport is slowed by changing the presynaptic Cl concentrations (Nakakubo et al., Figure 5A)

Biol Trace Elem Res (2014) 161:27287

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