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Such systems biology perspectives not only drive fundamental understanding of comorbidity mechanisms but also provide a theoretical foundation for developing next-generation trans-organ therapeutic strategies targeting systemic immunometabolic homeostasis

Add 0.25% trypsin (GIBCO, 15050065) and 1 mg/mL DNase I (Rubio, D3212, China) without EDTA in the collection of collagenase IV containing cells, continue digestion for 5 min, and then add 20% fetal The digestion was terminated by EGM-2 medium (LONZA, cc-3156 & cc-4176, USA) of bovine serum (FBS

Blood was withdrawn by retro-orbital sinus puncture through the medial canthus of the eye using glass capillaries

10.1038/nrendo.2010.171