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doi: 10.1002/14651858.CD005195.pub4 214 VineisP.PirastuR

We wont hit everything, which is why you need to read the book

PloS One 9 , e101840 (2014)

The CRISPR/Cas9 system was designed and executed in collaboration with Sunnybiotech, which carried out the sgRNA design, plasmid construction, microinjection, and screening for precise gene deletion