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The gene expression level was normalized to the level of GAPDH (Forward: 5-ACCCACTCCTCCACCTTTGA-3 and Reverse 5-TGTTGCTGTAGCCAAATTCGTT-3) and determined using the C T method

The glucose concentration was estimated by glucose oxidase-peroxidase (GOD-POD) method using commercially available kits (Coral Clinical System, Goa, India) [15]

Patients are advised to remain adequately hydrated before and after the procedure, follow all medical instructions carefully, and complete the prescribed treatment course for optimal therapeutic outcomes