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with (1R)-[1,1-binaphthalene]-2,2-diol Catalogue No.:PA 12 0301006 Molecular Formula : C 2 6 H 2 9 Cl 2 NO 3 Molecular Weight : 474.42 Propionylcarnitine Catalogue No.:PA 12 0301007 Molecular Formula : C 1 0 H 1 9 NO 4 Molecular Weight : 217.27 ()-Propionylcarnitine Chloride Catalogue No.:PA 12 0301008 Molecular Formula : C 1 0 H 2 0 ClNO 4 Molecular Weight : 253.72 L-Carnitine-d9 Catalogue No.:PA STI 089212 Molecular Formula : C 7 H 6 D 9 NO 3 Molecular Weight : 170.26 Isovalerylcarnitine Catalogue No.:PA 12 0301009 Molecular Formula : C 1 2 H 2 3 NO 4 Molecular Weight : 245.32 Decanoyl-L-carnitine-d3 Chloride Catalogue No.:PA STI 089282 Molecular Formula : C 1 7 H 3 1 D 3 ClNO 4 Molecular Weight : 354.93 D-Camphoric Acid Catalogue No.:PA 12 0301011 Molecular Formula : C 1 0 H 1 6 O 4 Molecular Weight : 200.23 trans-Crotonobetaine Catalogue No.:PA 12 0301019 Molecular Formula : C 7 H 1 3 NO 2 Molecular Weight : 143.19 (2R)-3-Hydroxyisovaleroyl-d3 Carnitine Catalogue No.:PA STI 090233 Molecular Formula : C 1 2 H 2 0 D 3 NO 5 Molecular Weight : 264.34 Acetyl-L-carnitine-d9 Catalogue No.:PA STI 090214 Molecular Formula : C 9 H 8 D 9 NO 4 Molecular Weight : 212.29 Stearoyl-L-carnitine-d3 Catalogue No.:PA STI 090217 Molecular Formula : C 2 5 H 4 6 D 3 NO 4 Molecular Weight : 430.69

HuR binds to GLS transcripts and influences the isoform levels To confirm that HuR impacts GLS isoforms, both at the protein and mRNA levels, we knocked down ELAVL1 in 6 breast cancer cell lines (SKBR3, BT549, MDA-MB-231, MDA-MB-157, HCC38, and Hs578t)