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Insert the needle swiftly into the fold of skin, with the needle angled downwards at a thirty- to forty-five-degree angle

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To identify the selective cargos of V-ATPase-dependent autophagy, we tagged different organellar proteins with GFP at the C-terminal end and performed GFP-processing assays: We found a clear increase in the vacuolar degradation of ribosomal proteins Rps6A and Rpl10, indicating the induction of ribophagy (Fig

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