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Ultimately, maturation of mDA neurons is marked by the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) in dendrites and soma, VMAT and SYN1 expression in the vesicle of synaptic terminals, the evidence of functional release and uptake of DA and the repetitive burst firing, a signature of electrophysiological DA neuron phenotype (Weihe et al., 2006)

Ancient DNA (aDNA) supports these timelines: selection tests on aDNA show that variants in FADS were selected before and after the agricultural revolution in Eurasia (59)

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