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Scenario 1: Adding 1ml of Bacteriostatic Water This is the most common method because the math is incredibly simple

Stockwells lab in Columbia University report that the gene expression of CHAC1, PTGS2, SLC7A11 , and ACSL4 was induced during ferroptosis, and their upregulation detected by qPCR can be regarded as an indicator of ferroptosis (Dixon, Patel et al., 2014, Stockwell, 2022)