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Each GFM consisted of a slurry of 8 g of wheat flour (section Enzyme-linked immunosorbent assay quantification of gluten immunogenic peptides) in 80 mL of simulated salivary fluid (Brodkorb et al, 2019), supplemented with 10 mL of 20 mM TrisHCl pH 7.5, 150 mM sodium chloride, and either no Clc (vehicle), Clc at 0.16 mg/mL (at 1:250 enzyme:gliadin ratio), or Clc at 0.80 mg/mL (1:50) (Fig