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Genome-wide analysis of nucleotide-level variation in commonly used Saccharomyces cerevisiae strains

Iron overload-induced programmed cell death The academic community has consistently recognized the mechanisms related to increased mitochondrial oxidative stress and impaired energy metabolism in the context of iron overload

For the reverse transfection on a Freedom EVO 200 workstation (Tecan), 5 l of 250 nM siRNA per well, diluted in Opti-MEM (Thermo Fisher Scientific), were placed into a 384-well PhenoPlate (PerkinElmer) followed by the addition of 0.02 l RNAiMAX (Thermo Fisher Scientific) in 5 l Opti-MEM using a BioTek EL406 Washer Dispenser (Agilent)

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