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In vitro palmitoylation of GSTP1 was markedly reduced when the protein was treated with NEM at pH 7 (blocks Cys) before subjecting GSTP1 to in vitro palmitoylation and further reduced when treated with NEM at pH 10 (blocks Cys and Lys), suggesting that in vitro palmitoylation occurs through a thioester and amide bond or that Cys residues are required for the acylation onto a final Lys acceptor (e.g., Lys103)

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Consult with your physician before discontinuing any medications

M., Lorini A., Antunes B