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In LPS-stimulated HMC-3 microglia, Western blotting showed marked mTORC1 activationas indicated by increased p-mTOR, p-S6K1, and p-4E-BP1all of which were suppressed by butyrate but not by ISRIB (Fig

In vitro experiments were performed in human macrophage cell line THP1 cells stimulated with conditioned medium derived from human liver epithelial cells (THLE-3) and HCC cell lines (HepG2 and Huh7)