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ELISA for total IgG quantification Total serum IgG concentrations were measured using a commercial mouse IgG ELISA kit (PI480, Beyotime, Shanghai, China) according to the manufacturers protocol with minor adaptations

However, much of the research on BPC-157 has been conducted in animals, and key questions about safety, effectiveness, dosing, and long-term outcomes remain unanswered

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These findings highlight the necessity of systematic toxicological assessments and long-term clinical follow-ups, especially in vulnerable populations, such as children, pregnant women, and patients with chronic illnesses