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The membrane was blocked at room temperature with TBST (5 mL) containing 5% skim milk (P0216-300 g, Beyotime) for 1 h and incubated with the primary antibody overnight at 4

J Nutr 134(3):489492 Yae T, Tsuchihashi K (2012) Alternative splicing of CD44 mRNA by EsRP1 enhances lung colonization of metastatic cancer cell

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184 NAD + manipulates the gene expression Cellular metabolism, such as NAD + metabolism, is directly connected to gene expression through regulating the post-translational modifications (PTMs) of histones, the covalent chemical modifications of DNA, the activity of transcription factor and RNA processing