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The crossing of these cellular barriers is essential for the development of the IMD, and although it has been intensively studied, it remains not fully understood

Prior to metabolomic analysis, samples were homogenized in 1 mL of pre-chilled (80 C) 80% methanol containing isotopic internal standards using a high-throughput bead-mill homogenizer

Unlike an additive effect (1+1=2), synergy produces a result of the type 1+1=3

Int J Plant Sci 161:861868 Hothorn M, Wachter A, Gromes R, Stuwe T, Rausch T, Scheffzek K (2006) Structural basis for the redox control of plant glutamate cysteine ligase