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Cell culture and treatment AML-12 cells, a nontumorigenic mouse hepatocyte cell line, were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and cultured in DMEM/F12 containing 0.005 mg/ml insulin, 0.005 mg/ml transferrin, 5 ng/ml selenium, 40 ng/ml dexamethasone and 10% FBS at standard cell culture conditions (5% CO 2 , 95% air)

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These often contain unapproved ingredients that may affect liver or kidney function