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Key representatives include gallic acid (GA) (a hydroxybenzoic acid found in berries, tea, and other sources) and caffeic acid (a hydroxycinnamic acid abundant in coffee, apples, and herbs), along with others like ferulic and p-coumaric acids (148)

Then, the UV detector monitors the eluent at 260 nm absorbance to extract the SEC fraction containing both unbound RNA and RNA-ligand complexes

GLS1 is inhibited by its product glutamate

Advantages offered by these materials are their availability in a variety of pore sizes and particle diameters, their good mechanical strength under the conditions used in HPLC systems, their ability to be modified for the immobilization of a wide range of affinity ligands, and their compatibility with applications that spanned from target purification to pharmaceutical or biomedical analysis and flow-based immunoassays [4,10,4547,5153]