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From tartaric acids Four-carbon chirons derived from D- or L-tartaric acids can be used as starting materials in syntheses of enantiomers of 3,4-dihydroxyglutamic acids since they contain a vicinal diol fragment of the known stereochemistry

Protocol [1] A total of two sets of adult male C57BL/6j mice weighing 21-26 g are randomly assigned to one of four groups and treated with intraperitoneal (i.p.) delivery of (i) vehicle (50 L isotonic saline/5% ethanol), (ii) melatonin (4 mg/kg, dissolved in 0.9% isotonic saline/5% ethanol), (iii) Wortmannin, and (iv) melatonin/Wortmannin immediately after reperfusion