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After the cells reached ~ 80% confluence under normal culture conditions, the medium was changed to glucose-free DMEM (Gibco) and the dish was placed in an anaerobic chamber (5% CO2, 95% N2) to induce OGD-related cell injury

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High-throughput lipidomics/metabolomics may yield biomarkers reflecting disease activity, predicting treatment response, or distinguishing patient subtypes, enabling early diagnosis, personalized therapy, and treatment monitoring

For this, we decreased the levels of glutathione, which is a key antioxidant molecule in the cell