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Description

Measure of competitive binding titers by ELISA Coating, blocking, and gH/gL immobilization steps were performed as described under Measurement of serum antibody endpoint binding titers by anti-His capture ELISA. Following the capture of monomeric gH/gL, equal amounts of sera from each mouse in a group were pooled and diluted in blocking buffer and 2-fold serial dilutions were performed, followed by a 1 h incubation at 37 C