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For confocal microscopy, BMDM were treated with or without 250 M palmitate for 24 hours and incubated with 200 nM mitotracker green, 200 nM mitotracker deep Red (Excitation/emission: 644/665, in the Cy3 channel), and 5 M of mitosox red (PE), and counterstained with 300 nM DAPI (Invitrogen, #D1306) for 30 minutes in PBS

Importantly, PCs can synthesize precursors of inflammation-inducing substances 53 that are released and subsequently produce modulatory oxidative metabolites implicated in the pathogenesis of ARDS

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A tiny drop of medication at the injection site after withdrawal is normal and represents an insignificant amount