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Cd44 variant regulates redox status in cancer cells by stabilizing the xct subunit of system xc and thereby promotes tumor growth

2 L of samples were injected on a Waters XBridge XP BEH Amide column (150 mm length 2.1 mm id, 2.5 m particle size) maintained at 25 C, with a Waters XBridge XP VanGuard BEH Amide (5 mm 2.1 mm id, 2.5 m particle size) guard column

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For better accuracy, we also used unstained proteins as molecular weight markers, detected after transfer to PVDF by Ponceau Red staining (not shown)