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Notably, studies in rats indicated that dynamic PEMF (DPEMF) stimulation is more effective than static PEMF (NPEMF) stimulation in alleviating IDD (Zheng et al., 2022a)

Lysates were incubated with glutathione sepharose 4B (Cytiva, 17-0756-1) or Ni 2+ -NTA beads (Qiagen, 30210) overnight at 4 C with gentle rotation and the beads were washed with lysis buffer

Its low toxicity and irritancy to the eye, biodegradability, biocompatibility, and bioadhesive characteristics make it an ideal candidate for developing CS-based NPs as novel DDSs [137]