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Patients often report a gradual but positive change over weeks of therapy

To map the region of the human PPARGC1A promoter that is responsible for the inhibitory effect of PPAR on PPAR-mediated upregulation of PPARGC1A expression, we generated a panel of PPARGC1A promoter deletion mutants (Supplemental Figure 4C) and cloned this panel into the pGL3-BV luciferase reporter plasmid