The separated proteins were transferred to a nitrocellulose membrane and incubated with primary antibodies against GLUT3 (Abcam, ab191071, EPR10508(N), or ab15311), GLUT1 (MilliporeSigma, 07-1401), p-STAT1 (Y701) (Cell Signaling Technology, 7649, D4A7), STAT1 (Cell Signaling Technology, 14994, D1K9Y), p-STAT6 (Y641) (Cell Signaling Technology, 9361), STAT6 (Cell Signaling Technology, 5397, D3H4), p-JAK1(Y1034/1035) (Cell Signaling Technology, 3331), JAK1 (Cell Signaling Technology, 3332), Na + /K + -ATPase (Cell Signaling Technology, 3010), EEA1 (Cell Signaling Technology, 48453, E9Q6G), IL-4R (OriGene Technologies, AP20570PU-N), c chain (R&D Systems, AF284), p-PAK (PAK1[T423]/PAK2[T402]) (Cell Signaling Technology, 2601), PAK1 (Cell Signaling Technology, 2602), pan-RAS (Cell Signaling Technology, 8955, D2C1), GST (Santa Cruz Biotechnology, sc-138, B-14), and Hsp90 (Cell Signaling Technology, 4877)

TTLL6, gold, binds every 80 , highlighted by the double arrow, along the microtubule lattice, blue
10.1016/j.tcb.2012.04.006 21 SantoroM
Interestingly, the triple mutant exhibited increased sensitivity to excess Fe, suggesting that chloroplastic ferritins may actually act as Fe scavengers