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Some scholars have proposed that TBI led to gastrointestinal tract damage, which in turn drives immune reactions and systemic inflammation which further exacerbates neuroinflammation (9, 10)

Acetyl L-Carnitine: Carnitine helps transport fatty acids into the mitochondria of muscle cells, promoting increased energy production and potentially aiding in lactic acid removal

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iHLOs were then incubated with the primary antibody solution diluted in antibody dilution buffer (Biolegend, catalog: 427708) overnight at 4 C with shaking