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Furthermore, while in 2D/unconfined environments breast cancer cells maintain a mesenchymal migration phenotype characterized by actin-rich protrusions and adhesion to the substrate [7], in confinement, cells switch to an amoeboid/bleb-based migration mode [152, 164, 165]

When using dilute acetic acid (0.1% v/v) as a solvent, it can improve initial solubilization of certain peptide batches, but the solution pH should be adjusted to near-physiological range before administration to avoid tissue irritation at the injection site in animal models