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Importantly, activation of NOX generates the substrate for the reaction catalyzed by SOD1 (Mccord and Fridovich, 1969)

Inhibitors 6 - 8 were incubated with TGR with and without NADPH, and the inhibition of TGR activity was determined after adding a second aliquot of NADPH and 5,5-dithiobis (2-nitrobenzoic acid) (DTNB)