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Padayatty et al., 2016)

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Consistent, restorative sleep helps maintain Glutathione balance, while chronic sleep disruption depletes this vital molecule

Immunoprecipitation was performed with Flag agarose M2 affinity beads (Sigma-Aldrich, A2220, 15 l per sample) or with BUD13 antibody (A303-321A) prebound to protein G Dynabeads beads (20 l per sample)