glutathione resin protein purification Sepharose 4B GST-tagged resin, 300 mL-CYTIVA 17075604 How to Regenerate Glutathione Resin

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Fer-1 (Glpbio, GC10380) was initially dissolved in dimethyl sulfoxide (Sigma, D2438) and diluted with 0.9% NaCl solution (Beyotime, ST341) to achieve a final concentration of 0.25 mg/mL Fer-1 and 0.1% DMSO

(2) Downstream protection with cartilage-building substrates (e.g., collagen peptides, vitamin D) to enhance matrix integrity (11, 12)

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