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Gels were homogenized with a polypropylene EPPI-pestle homogenizer in the eppendorf tube for 10 to 20s at room temperature until most of the homogenate pieces were small enough to pass through a 1 mL micropipette tip

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To investigate whether MAT2A serves as a critical regulator of SAM homeostasis and lipid metabolism, MAT2A protein expression in HT1080 cells under essential amino acid deprivation was first examined