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Total Cholesterol (TC) 0.5ml of working reagent was added to a set of clean labeled test tubes and two of the test tubes were labeled standard 1 and 2 while another was labeled blank.0.1ml of homogenate was added to the approximately labeled test tube .mixed and incubated for 30 minutes at 37, the absorbance of the sample and standard were read against the reagent blank at the wavelength of 546nm

65-year-old patient Enalapril + Furosemide Diclofenac added for pain Within days: Urine output dropped Creatinine spiked Diagnosis: This is the ACE inhibitor + Diuretic + NSAID Individually

GLP-1 agonists reduce caloric intake, which drives weight loss but does not specifically target visceral or subcutaneous fat