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Furthermore, it remains unknown whether increasing GPx1 expression in macrophages derived from patients with CD (either genetically or pharmacologically) would reduce their susceptibility to H 2 O 2 -induced cell death to levels comparable to that in healthy macrophages

Studies have found that nearly half of patients with unexplained cholestatic liver disease after standard diagnostic workup had detectable gene variants for PFIC mutations in the ABCB4 and ABCB11 genes

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All primary antibodies (1:1000 in TBST, overnight at 4 C) incubations were followed by incubation with secondary HRP-conjugated antibody (1:5000 in TBST, 1 h at room temperature), and then visualized using chemiluminescent HRP substrate on chemiluminescence image system (Tanon Science and Technology Company)