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D.RudinC

Each woman with PCOS can have a different hormonal pattern and root causes, requiring a personalized treatment strategy

A., Iwata, W., Okumichi, H

A method of stabilising a biological sample comprising glutathione (GSH) and glutathione disulfide (GSSG), the method comprising the following steps: a) providing the biological sample comprising GSH and GSSG, b) contacting GSH and GSSG of the biological sample with a maleimide under conditions which allow alkylation of the sulfhydryl group of GSH with the maleimide to obtain maleimide-alkylated GSH, c) separating excess maleimide from maleimide-alkylated GSH and GSSG, d) contacting maleimide-alkylated GSH and GSSG with a reducing agent, selected from the group consisting of phosphine-based reducing agents such as tris(2-carboxyethyl)phosphine (TCEP) or tributylphosphine, and thiol-based reducing agents such as dithiothreitol (DTT), dithioerythritol (DTE) or -mercaptoethanol, under conditions which allow reduction of GSSG by the reducing agent to obtain further GSH, and e) contacting maleimide-alkylated GSH and GSH with a heavy isotopologue of the maleimide under conditions which allow alkylation of the sulfhydryl group of GSH with the heavy isotopologue of the maleimide to obtain a heavy isotopologue of the maleimide-alkylated GSH