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The most common mutation in IDH1 occurs at residue 132, where arginine is replaced by histidine (IDH1-R132H) [12, 13]

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A single pass of a serum or cell-lysate sample through an affinity column can achieve greater than 1000-fold purification of a specific protein so that only a single band is detected after gel electrophoresis (e.g., SDS-PAGE) analysis