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Consistent with this, the observation of reduced viability of H9c2 cardiomyoblasts and HUVEC endothelial cells to values below 80% when incubated with GSNO concentrations above 75 m and 156 m, respectively, indicates that the cytotoxic effect of high GSNO concentrations, such as 500 m, may prevail over the beneficial effect of cardioprotection in I/R