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Finally, soluble nuclear lysate was diluted with two volumes of the dilution buffer (60 mM HEPES-KOH (pH = 7.9), 1 mM DTT, 30 % glycerol, 0.4 % NP-40, 1 x phosphatase inhibitor cocktail (Sigma, P0044) and 1 x protease inhibitor cocktail (Sigma, P8340)) to lower KCL concentration to 150 mM

Low magnesium levels can contribute to insulin resistance, making blood sugar harder to control and increasing the risk of type 2 diabetes[6]

Using standardized reconstitution volumes simplifies the math and ensures consistency across the protocol