mucosal melanotic macule lip Mystery behind labial and oral macules: Clinical, dermoscopic and pathological aspects of Laugier-Hunziker syndrome a Melanotic macule at the

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Samples were permeabilized for 30 min in ice-cold relaxing solution with saponin (50 g/mL) gently stirred and washed 3 times with MiR05 medium (60 mmol/L potassium lactobionate, 0.5 mmol/L EGTA, 3 mmol/L MgCl 2 , 20 mmol/L taurine, 10 mmol/L KH 2 PO 4 , 20 mmol/L HEPES, 110 mmol/L sucrose, 1 g/L BSA, pH 7.1) at 4C

This is a dramatic simplification, of course, but it captures the essence of its function