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After two PBS washes, the cells were treated with RNase A (Gibco, Grand Island, NY) for 30 min at 4 C to remove RNA and ensure only DNA is stained with propidium iodide (PI) (Sigma-Aldrich)

Statistical analysis Statistical analysis was performed using GraphPad Prism 9

Why Researchers Use Pre-Blended Stacks Running four separate peptides in a research model introduces reconstitution variability, storage complexity, and potential for component-ratio inconsistency across experimental replicates

The efficacy of ferroptosis inhibitors in AD models reveals three key therapeutic development avenues: modulation of iron metabolism, suppression of lipid peroxidation, and restoration of GSH system functionality