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CN108567970B - Preparation method of reduced glutathione for injection - Google Patents Preparation method of reduced glutathione for injection Download PDF Info Publication number CN108567970B CN108567970B CN201810530808.9A CN201810530808A CN108567970B CN 108567970 B CN108567970 B CN 108567970B CN 201810530808 A CN201810530808 A CN 201810530808A CN 108567970 B CN108567970 B CN 108567970B Authority CN China Prior art keywords injection reduced glutathione temperature solution plate layer Prior art date Legal status (The legal status is an assumption and is not a legal conclusion

The resulting lysates were centrifuged at 4 C, 12,000 rpm for 30 min for Western blot experiments

Between 2019 and 2023, approximately 50% of the broccoli in the Canadian retail sector, household and service sector was wasted ( Broccoli senescence is associated with yellowing and loss of green chlorophyll pigmentation within the florets ( SAG12 ) is more accelerated in broccoli heads stored at 25C relative to those held at 4C ( 2 O 2 and the dysfunction of the ascorbate-glutathione (GSH) recycling pathway ( 2 O 2 and lose smaller proportions of ascorbate and GSH during 10C storage relative to untreated florets ( Ergothioneine (EGT) is a histidine-derived thiol antioxidant that is less prone to autooxidation than GSH ( 2+ and Cu 2+ that would otherwise mediate the conversion of H 2 O 2 to the more reactive hydroxyl radical ( Here we investigated the effect of partial submergence of broccoli in a solution of EGT (0 M, 20 M, 100 M or 500 M) on the dynamics of senescence of this inflorescence vegetable including floret yellowing when stored at 10C, and the relationship to shifts in oxidative stress metabolism

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