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doi: 10.4049/jimmunol.163.4.2209 120 HobbsJAMayRTanousisKMcNeillEMathiesMGebhardtCet al

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Proper reconstitution technique matters: Allow both the peptide vial and bacteriostatic water to reach room temperature before mixing Clean both vial stoppers with alcohol swabs Insert the needle at an angle and direct the stream of water against the glass wall, not directly onto the powder Gently swirl or roll the vial until the peptide dissolves completely Never shake vigorously, as this can damage peptide bonds The solution should be clear and colorless when fully reconstituted