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The increased abundance of lipoylated proteins, combined with the elevated labile copper pool induced by DOX, creates a permissive environment that drives cuproptosis

S., Previll L

If your experiment is designed to study GSH, then using a sample that has oxidized to GSSG will invalidate your results

After 48 hours of transfection, the cells were cultured in complete medium supplemented with 2 g/mL puromycin for 72 hours to select the successfully transfected cells