is glutamate part of glutathione Optimizing Glutathione's Component Amino Acids

1401708-83-5 Appearance Solid Molecular Weight 504.66 Formula C 27 H 44 N 4 O 5 Color White to off-white SMILES OC(C=C1)=CC=C1C[C@H](NC(CCCCC)=O)C(N[C@@H]([C@@H](C)CC)C(NCCCCCC(N)=O)=O)=O Synonyms PNB-0408

For the reconstitution of egtA AfS77 , a functional copy of egtA (a 5691 bp PCR-amplified fragment generated with the primers oAetgA1.7_f and oAegtA1.8_r) was subcloned into pAN8.1 containing the phleomycin resistance cassette ( ble ) by a digestion with Sph I and Nhe I resulting in (p)egtA:ble

PLoS ONE 9:e86834

Cells were centrifuged 5 min at 7,000 g , and pellets were resuspended in fresh oxic medium to obtain an initial OD 600 0.005