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Due to chemisorption, the sulfhydryl groups in glutathione can combine with silver nanoparticles to produce AgS bonds, which attach to the silver nanoparticles surface and generate a large number of hot spots, resulting in the enhancement of the infrared signal intensity of glutathione

Acknowledgements We thank the staff of the Division of Analytical Science, Hidaka Branch of the Biomedical Research Center, Saitama Medical University, for providing research equipment and offering important advice