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Control 3T3-L1 cells were generated using the same protocol but with a non-targeting sgRNA

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The skin in those areas will start looking smoother and more even

In recent years, efforts have been made to apply dynamic control of metabolism on the constraint-based approaches by taking extracellular by-products into account, leading to dynamic FBA (dFBA), through which dynamic models to study a bio-system under transient conditions can be developed to capture the dynamic changes in metabolic fluxes through time series of concentration measurements (Llaneras et al., 2012