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doi: 10.1002/mds.27372 32 FuY.ItoM.FujitaY.ItoM.IchiharaM.MasudaA.et al

The protective effect of inflammatory monocytes during systemic C

After drug treatments, the cells were fixed with 4% paraformaldehyde for 20 min and infiltrated with 0.2% Triton X-100 for 15 min, followed by blocking in donkey serum for 30 min and incubating overnight at 4C with primary antibody (GPX4, rabbit, ab125066, 1:200, Abcam) in PBS